Lindolirian is pretty close on this one I think. There is more than one quote concerning the ents and entwives in Letters. Tolkien seems to think the Entwives did not survive, as read here:
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I think that in fact the Entwives had disappeared for good, being destroyed with their gardens in the War of the Last Alliance (Second Age 3429-3441) when Sauron pursued a scorched earth policy and burned their land against the advance of the Allies down the Anduin (vol. II p. 79 refers to it2). They survived only in the 'agriculture' transmitted to Men (and Hobbits). Some, of course, may have fled east, or even have become enslaved: tyrants even in such tales must have an economic and agricultural background to their soldiers and metal-workers. If any survived so, they would indeed be far estranged from the Ents, and any rapprochement would be difficult – unless experience of industrialized and militarized agriculture had made them a little more anarchic. I hope so. I don't know.
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'"... all the gardens of the Entwives are wasted: Men call them the Brown Lands now."'
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Of course Tolkien does say a few may have survived. BUt what Sam was talking about was probably and Ent or Huorn(more likely) from the Old Forest. In LotR it does say that trees are constantly waking up(becoming entish) and falling back to sleep.